When you pour it off into a bucket to paint out of, you should give it a little stir. One thing I've learned is that if you pour the gallon into another bucket, and you see tint streaks on the side of your original bucket, rinse that bucket out. That way, when you pour your leftover paint back in, you know there wont be differences in paint.
I really dont worry too much about stirring my paint, although that is my own bad habit, so I dont encourage it. Generally, once the paint and tint is originally shaken, it'll stay good for a while, and you'll know it when its separated. I dont advise taking it back to the store, unless they actually forgot to shake it after they tinted it, which does happen every once in a while. I just dump it in a five gallon bucket and stir it from there.
That said, it is always better to overstir it, or take it to the store, than not to..